CAPSO Buffer 0.2M, pH 8.5

CAPSO (N-cyclohexyl-2-hydroxyl-3-aminopropanesulfonic acid) is a biological and zwitterionic buffer commonly categorized as a Good's buffers. CAPSO has many applications some of which include immunoblotting and westerns. Additionally, CAPSO is used in the electrotransfer of proteins to PVDF or nitrocellulose membranes. CAPS buffer has very low metal-binding constants and are, therefore, particularly suited to investigate metal-dependent enzymes. CAPSO a very water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins with minimal salt effects.